Total WBC Count / T-Leukocyte Count

Requirement :-

Reagent :- WBC Diluting fluid.

                    WBC Diluting fluid hemolysis the RBC due to acidity. So that the counting of white cells become easy. The following are the formula of turk solution -----

Calculation of Red Blood Cell indices

The quantative measurements of the average size by volume (V) hemoglobin content by weight (W) and hemoglobin concentration (W?V) of the Red Blood cells. Are of substance aid in diagnosis of various types of anaemias. For the manual procedure these various are calculated from the total number of Red cells. The hemoglobin concentration and the hematocrit . these are called red cell indices , three commonly used red cells indices are.  

Total Erythrocyte count (TRBC)

Blood pipette (Sahli’s) 50 micro liter and 20 micro liter

2)      Hemocytometer /Neubauer chamber

3)      Microscope with minimum 10x and 40x objective.

HEMATOCRIT ( Hct or PCV)

Hematocrit or packed cell volume is the amount of packed red blood cell following centrifuzation , expressed as a percentage of the total blood volume. Two method are available for determining hematocrit.

a)          Microhematocrit Method

b)          Macro hematocrit Method

 Specimen :-

 Only anti coagulated blood EDTA blood is used in determination Hct value.

Equipment :-

a)      Wintrobe hematocrit tube 110 mm long narrow test tube graduted from 0-10 cm(100mm). the scale with the marking in ascending order from the to is used for ESR determination while the scale with descending order is used for Hct determination

b)     Transfer pipette a long fine capillary pasteure pipette

c)      Centri fuge

Procedure :-

1)      The blood specimen is mixed carefully

2)      The wintrobe tube is marked labeled  with the identify number of the patient

3)      The tube is filled with blood specimen by the help of pasteure pipette up to the 10 cm marked which blood or taken as blank to balance the centrifuge

4)      The wintrobe tube are placed in the opposite cups of the centrifuge

5)      The centrifuge is turned on to slow speed the increase gradually to bring up finally the required speed.

6)      The tube are centrifuge for 30 minutes at 3000 rpm ( Revolution per minute)

7)      After 30 minute centrifuge is switched off and left it self to stop spilling.

8)      The wintrobe tube is taken out and observed that the blood sample has been  lower portion of tube and plasma above. It the amount of packed RBC are read directly from the graduation mark of the tube.